beta ngf elisa kits  (Proteintech)

Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet: MSM-DTM adsorbs inflammatory cytokines, suppresses TSPCs inflammation and senescence, and promotes tenogenic differentiation and migration (A) The remaining concentrations of TNF-α, IL-1β, IL-6, IFN-γ, LPS, and NGF were detected by ELISA after co-culture with different concentrations of MSM. (B and C) RT-qPCR of IL-6 and CCL-2 in TSPCs under different treatments. (D) Western blot of COL1A2, MMP3, and TNMD. (E) ELISA of TNF-α, IL-6, and IFN-γ in supernatants. (F)Western blot of p16 and p21 after 7 days of treatment. (G) β-Galactosidase staining analysis after 7 days of treatment. Scale bars, 100 μm. (H and I) Immunofluorescence and quantification of TNMD and TNC after 14-day treatment. Scale bars, 50 μm. (J) Relative mRNA expression of tenogenic differentiation markers SCX and TNC. (K and L) Representative images and quantification of Transwell assay. Scale bars, 100 μm. (M and N) Representative images and quantification of wound healing assay. Scale bars, 100 μm. Statistical comparisons were performed with one-way ANOVA with Tukey’s multiple comparisons test. Data are presented as the mean ± SD, n = 3. Ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 between groups.

Article Snippet: The remaining cytokines in the supernatant were quantified using ELISA kits for TNF-α (ABclonal, China), IL-1β (ABclonal, China), IL-6 (ABclonal, China), IFN-γ (ABclonal, China), and NGF (ABclonal, China), and LPS (ABclonal, China), following the manufacturers’ protocols.

Techniques: Migration, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Quantitative RT-PCR, Western Blot, Staining, Immunofluorescence, Expressing, Transwell Assay, Wound Healing Assay

Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet:

Article Snippet: The remaining cytokines in the supernatant were quantified using ELISA kits for TNF-α (ABclonal, China), IL-1β (ABclonal, China), IL-6 (ABclonal, China), IFN-γ (ABclonal, China), and NGF (ABclonal, China), and LPS (ABclonal, China), following the manufacturers’ protocols.

Techniques: Recombinant, Lysis, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Staining, Software

Journal: CNS Neuroscience & Therapeutics

Article Title: hUC ‐ MSCs via β‐ NGF Alleviate Cognitive Impairment After Tibial Fracture Surgery by Regulating the STMN2 / NMNAT2 ‐ SARM1 ‐ NF ‐ κB Signaling Pathway

doi: 10.1002/cns.71062

Figure Lengend Snippet: hUC‐MSCs exert therapeutic effects in the PND mouse model via β‐NGF. (A) 20‐Human Neuro Discovery array analysis between blank‐medium and MSC‐conditioned medium (MSC‐CM) (B) ELISA quantification of β‐NGF levels in blank‐medium and MSC‐CM ( n = 3). (C, D) Representative Western blot bands and quantitative analysis of β‐NGF protein expression in hippocampal tissues from CON, CON‐MSC, SUR, and SUR‐MSC groups. (E) Total distance traveled by mice in OFT ( n = 9). (F) Alternative corrections (%) in Y‐maze test ( n = 9). (G) Novel object preference (%) in NOR ( n = 9). (H) Representative GFAP, NeuN and IBA1 immunostaining images of hippocampal CA1 region. (I) Representative Golgi staining images (J) Quantitative analysis of NeuN positive area percentage in hippocampal CA1 region ( n = 6). (K) Quantitative analysis of GFAP positive area percentage in hippocampal CA1 region ( n = 6). (L) Quantitative analysis of IBA1 positive area percentage in hippocampal CA1 region ( n = 6). (M) Quantitative analysis of dendritic spine density in hippocampal CA1 region. Representative Western blot bands (N) and quantitative analysis of β‐NGF (O), p‐TrkA/TrkA (P), STMN2 (Q) and NMNAT2 (R) protein expression in hippocampal tissues from CON, SUR, SUR‐MSC NC and SUR‐MSC NGFsiRNA groups ( n = 6). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The supernatant was harvested following the manufacturer's instructions, and the concentrations of IL‐1β (HUABIO, China, EM0001), IL‐6 (LIANKE, China, EK206), TNF‐α (HUABIO, China, EM0010), mouse (HUABIO, China, RK00154), and human β‐NGF (Abclonal, China, RK03064) were quantified using ELISA kits.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Immunostaining, Staining

Journal: Journal of Advanced Research

Article Title: Targeting NGF but not VEGFR1 or BDNF signaling reduces endometriosis-associated pain in mice

doi: 10.1016/j.jare.2024.08.017

Figure Lengend Snippet: Levels of VEGFR1 ligands are increased in the peritoneal fluid of endometriosis patients and in mouse lesions. (A) experimental procedures for peritoneal fluid collection from endometriosis patients. (B) The levels of VEGFA, VEGFB, PlGF, and sVEGFR1 in peritoneal fluid samples determined by Ella®. Data are presented in box and whisker charts representing the levels of each mediator in pg/mL. (C) Calculated VEGFR1 occupancy as determined by previously published dissociation constant (K d ) and half maximal effective concentrations (EC 50 ) , , . Note that in published data, occupancy is approximately proportional to signalling . (D) Experimental procedures and timepoints for tissue collection in our mouse model. (E) 56 days after endometriosis induction, mouse lesions were collected for determination of VEGFA levels by ELISA. Results are presented as mean ± SEM of VEGFA levels, n = 10 (sham – uterine horn tissue), and n = 9 (endo) mice per group (*P<0.05 vs. sham by two-tailed Student’s t -test). (F) Profile of VEGFR1 expression in DRG nociceptors determined by immunohistochemistry 56 days after endometriosis induction.

Article Snippet: The concentration of NGF-β from patients’ peritoneal fluid and mouse endometriosis lesions was determined using a commercially available rat beta-NGF DuoSet ELISA kit (Cat# DY556, R&D systems, Minneapolis, MN, USA).

Techniques: Whisker Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Expressing, Immunohistochemistry